Osteochondritis dissecans refers to the separation of osteochondral fragments from the bed of the joint surface. Definition of lesion goes by the existence of an open epiphyseal line in a juvenile form and the absence, representing an adult form. The treatment of osteochondritis dissecans depends on age, on the epiphyseal line, its length, location and stability of the lesion. A conservative treatment is preferred in juvenile cases; a surgical intervention may be necessary for unstable lesions and those not responding to conservative approaches. Showing no positive development in symptoms and physical status for six months, our 16-year-old male patient received two intraarticular platelet rich plasma injections three months apart, focusing on the lesion of osteochondritis dissecans localized in the right knee. At the end of the 18th month, the patient did not show any limitation in his physical activities and radiographic examination confirmed the successful treatment. Having obtained positive results, we believe that PRP injections are a safe, simple, and minimally invasive treatment option for juvenile OCD which doesn’t respond to conservative therapy before a surgical approach.
Journal of Prolotherapy. 2016;8:e966-e970.
Osteochondritis dissecans (OCD) is a disease characterized by the separation of an osteochondral fragment from the bed of its joint surface due to impairment in the blood supply of the subchondral bone.1,2 Although its exact etiology is still unknown, acute or repetitive microtrauma, ischemia, genetic and endocrine pathologies have been implicated as etiologic factors.1,3,4 There are two main types of OCD, juvenile and adult, occurring before and after the closure of the physis, respectively.2,5 Its incidence increases as children get more involved in regular combat sports and peaks at the ages of 15 to 20 years.1,3 OCD mostly occurs in knee joints, with typical location in the lateral aspect of the medial femoral condyle.2-4 Although direct radiographs are sufficient for its diagnosis, magnetic resonance imaging (MRI) provides valuable information for the assessment of integrity of the joint cartilage, size and stability of the osteochondral lesion, the prognosis of the disease as well as for determining the choice of treatment.1-4,6 Its treatment varies depending on the patient’s age, complete or incomplete closure of the physis, and the size, location, and stability of the lesion.1,2 The juvenile form is likely to respond to conservative treatment methods.1,2,4-6 However, surgical procedures may be required when conservative treatment fails or in the presence of an unstable osteochondral fragment.2,4 Research has shown the healing rate for conservative treatment after 6-months to be only 50%.7 Platelet-rich plasma (PRP) is blood plasma, enriched with platelets and growth factors. It shows healing effects on impaired tissues of the musculoskeletal system and speeds up the regenerative process.8,9 In-vivo studies on the effects of PRP on cartilage repair in the knee joint were mostly related to degenerative cartilage and osteoarthritis.9 The use of PRP in OCD lesions was mainly limited to the ankle joint9 as our literature search provided no data referring to the application of PRP for OCD lesions of the knee joint. On the grounds of the effectiveness of PRP on injured tissue and due to it’s promising outcome in in- vitro and in-vivo studies, we decided to proceed with PRP injections in order to improve our patient’s comfort and quality of life. In this case report, clinical and radiographic results of an adolescent patient were presented in whom OCD of the knee joint did not respond to conservative treatment. PRP injections, however, lead to complete recovery and total healing of his lesion.
A 16-year-old male patient presented to our hospital with a six-month history of pain in his right knee, increasing in severity over time, after having encountered a traumatic fall in a football match 6 months prior. He reported increasing pain especially with walking long distances and physical activities, and although rare, accompanied by a sense of stumbling, friction and limping. The patient had no chronic disease, had no previous surgeries before and presented no family history. Six months before visiting our hospital, he sought treatment at another facility for similar symptoms. The preliminary diagnosis of osteochondral lesion of the knee joint had led to a conservative treatment, given the patient’s age and his juvenile form of OCD. The patient received a rehabilitation program, including stretching and strengthening exercises for his knee muscles. On physical examination, full active and passive ranges of motion, but pain along the medial border of his patella at the final stages of passive knee flexion were observed. Palpation over the medial femoral condyle during extension of the knee at 90° of flexion while the cruris was internally rotated casued pain. Anterior- posterior and lateral radiographs of the right knee and a tunnel X-ray at 30° of knee flexion showed no cartilage- bone lesions. The MRI showed an osteochondral lesion measuring 12.5 mm x 7 mm in the medial-lateral plane on coronal T2-weighted images (See Figure 1A.), and 21 mm x 8 mm in the anterior-posterior plane on sagittal images. (See Figure 1B.) Between osteochondral lesion and underlying subchondral bone, T2-weighted images showed hyperintense fluid, and T1-weighted images (See Figure 1C.) presented irregularities without significant splitting of the cartilage surface. According to the Dipaola MRI classification,10 the lesion was classified as grade III. His functional knee score was calculated as 34 according to the International Knee Documentation Committee (IKDC) scoring system.11 To predict the lesion’s healing probability and prognosis, a nomogram was produced, as described by Wall et al.12 (See Figure 2.)
The healing probability of the lesion by conservative treatment was estimated to be 50%. After a six-month follow-up of conservative treatment, there was no improvement in findings of physical status but also no deterioration. Therefore, treating with PRP injections was chosen. After drawing 30 ml of venous blood from the patient and centrifuging the sample at 3200 rpm for 10 minutes on a centrifugation device (Vision Scientific Co. Ltd., VS 400, Korea), 5 ml of PRP serum obtained manually13 was injected into the knee joint. Following the procedure, the patient was advised to refrain from strenuous physical exercise without restriction in daily activities. In addition, he was shown and instructed to perform exercises for strengthening his quadriceps and stretching his hamstring muscles. After monthly controls following the injection, radiographic and clinical assessments were repeated at the end of the 3rd month. The patient reported that, although he had no discomfort in daily activities, he experienced pain after long walks, less severe than before. On clinical evaluation, his IKDC score increased to 74. His follow-up MRI showed decreased subchondral edema around the lesion. (See Figure 3.)
The lesion decreased to a size of 8.75 mm (depth 6 mm) in the medial-lateral plane on frontal T2-weighted images, and to 13 mm (depth 6 mm) in the anterior-posterior plane on sagittal images. A subsequent PRP injection (5 ml) was administered 3 months after first injection. After another three months of follow-up, the patient reported complete resolution of his knee pain, without any functional limitation. At this stage, the IKDC score was 91 and he was cleared to return to his sportive activities. On the 18th month after his first PRP injection, the IKDC score was 100. The patient reported no restriction in his physical activities and that he started playing tennis regularly again. The final MRI and radiographic examinations showed no boundaries specific to the lesion. (See Figure 4.) During his last visit, written informed consent was obtained from the patient and his parents for this case presentation.
Healing rates of juvenile OCD lesions with conservative methods range from 50% to 67% in various reports.12-16 Uppstrom et al. report a 50% chance of healing with conservative treatment and refer to nomograms in order to calcualte the possible percentage of healing.7 These treatment methods include analgesic and anti- inflammatory medications, restrictions in sportive activities with modifications, brace or splint-cast immobilization, and physical therapy and rehabilitation protocols.4,5,17 The osteochondral lesion is considered stable when the diameter is less than 2 cm in open epiphysis plates, if continuity of articular cartilage is intact, and if no cystic lesion is present in subchondral area. A high likelihood of spontaneous healing was observed in stable lesions located on non-weight-bearing surfaces.1,2,4-6 Evaluation of the prognosis of OCD lesions should not only include the age of the patient and the skeletal maturity but also the size, the localization, and imaging features of the lesions. Apart from depicting localization, size, and relationship of lesions with intact surrounding tissues, MRI provides valuable information as considering a separation of the lesion from its bed and the responce to conservative methods.16,18 O’Connor, et al.18 asserted a high-density signal line indicating fluid accumulation behind osteochondral fragments, exhibiting an association with fissures or defects in articular cartilage on T2-weighted images, and showing normal density on gadolinium-enhanced images suggesting tendency for osteochondral part to separate from its bed. The authors advise examining T1-weighted images stating, if T1- weighted images show a split of cartilage, high-density signal line on T2-weighted images denote instability with 45% to 85% possibility. Contrary, some authors proposed the increased intensity signal behind the lesion on T2-weighted images being accounting for presence of vascular granulation tissue, thus suggesting healing of lesion.2,6 Yoshida, et al.6 noted spontaneous healing in nine of 16 patients with high-density fluid line behind the osteochondral fragment and proposed the high-density dual-line behind fragments being used as evidence of separation. Additionally, the presence of large homogeneous line below the lesion greater than 5 mm and a focal articular cartilage defect or a subchondral cyst greater than 5 mm were defined as instability criterion.2 In our case, to predict non-operative healing potential of the OCD lesion, we used nomogram developed by Wall, et al.12 using a regression analysis method. This measurement incorporates normalized values obtained from division of sagittal length and frontal width of the lesion by the diameter of the epiphysis on the same MRI images and the patient’s symptoms. After the insuffieciency of the six-month conservative treatment, MRI-based calculation yielded a 50% healing probability of the osteochondral lesion in our case. The increased signal intensity behind the lesion on T2-weighted images and irregularities on the cartilage surface on T1-weighted images were considered to significantly reduce the chance of improvement by conservative treatment. As the patient’s age gets closer to adulthood, the sooner the epiphyses will close, causing the length of conservative treatment to be shorter, and lowering the likelihood of non-operative healing.4,5 In cases of OCD lesions becoming unstable or conservative treatment failing, the lesion may separate from the cartilage bed, turn into a free fragments, and trigger a degenerative process within the knee joint. The need for surgical intervention has been emphasized in such cases,2,4 including arthroscopic transarticular or retroarticular drilling, microfracture surgery, internal fixation of the fragment, mosaicplasty or osteochondral allograft transplantation, autologous chondrocyte implantation, and fragment excision. Although success rates of surgical procedures range from 75% to 100%5, lesions unresponsive to conservative treatment, another treatment option before surgery may be less invasive methods. Platelet-rich plasma contains growth factors (TGF-beta, PDGF, FGF, EGF, VEGF, IGF-1, etc.) stored in alpha granules within platelets and play an important role in tissue healing. Injections of PRP allow factors to act favorably on angiogenesis of damaged tissues and control local inflammation, resulting in inhibition of catabolic enzymes and cytokines, accumulation of stem cells and fibroblasts in damaged tissues, proliferation of chondrocytes, and stimulation of type II collagen and proteoglycan synthesis, accelerating healing process.8,9,19 The use of PRP injections for osteochondral lesions is often accompanied by arthroscopic applications expediting the healing process.19 No other case of OCD improved solely after PRP injections following failure of conservative treatment and without needing surgery has been reported.
The main objective of OCD treatment is ensuring union of the lesion with the underlying subchondral bone without damaging the cartilage integrity, preserving the patient’s own cartilage. Compared to arthroscopic interventions, the PRP application is minimally invasive, safe, reproducible, and less expensive with very few to almost none adverse effects. In case of conservative treatment failing, intraarticular PRP injections can still provide rapid radiographic, clinical and functional improvement in OCD lesions encountered in adolescents and decrease the need for surgical intervention, as seen in our case.
1 Robertson W, et al. Osteochondritis dissecans of the knee in childrePubmen. Curr Opin Pediatr. 2003 Feb;15(1):38-44. [Pubmed]
2 Tandogan NR, et al. Osteochondritis dissecans. Acta Orthop Traumatol Turc Suppl. 2007;41(2):113-122. [Pubmed]
3 Kessler JI, et al. The demographics and epidemiology of osteochondritis dissecans of the knee in children and adolescents. Am J Sports Med. 2014 Feb;42(2):320-326. [Pubmed]
4 Schulz JF, et al. Juvenile osteochondritis dissecans of the knee: current concepts in diagnosis and management. Instr Course Lect. 2013;62:455-467. [Pubmed]
5 Eismann EA, et al. Management strategies for osteochondritis dissecans of the knee in the skeletally immature athlete. J Orthop Sports Phys Ther. 2014 Sep;44(9):665-679. [Pubmed]
6 Yoshida S, et al. Osteochondiritis dissecans of the femoral condyle in the growth stage. Clin Orthop Relat Res. 1998 Jan;346: 162-170. [Pubmed]
7 Uppstrom TJ, et al. Reliability of predictive models for non- operative healing potential of stable juvenile osteochondritis dissecans knee lesions. Knee (2016), http://dx.doi.org/10.1046/j. knee.2016.03.005. [Pubmed]
8 Foster TE, et al. Platelet-rich plasma: from basic science to clinical applications. Am J Sports Med. 2009 Nov;37(11):2259- 2272. [Pubmed]
9 Dold AP, et al. Platelet-rich plasma in the management of articular cartilage Pathology: a systematic review. Clin J Sport Med. 2014 Jan;24(1):31-43. [Pubmed]
10 Dipaola J, et al. Characterizing osteochondral lesions by magnetic resonance imaging. Arthroscopy. 1991;7(1):101-104. [Pubmed]
11 Schmitt LC, et al. Validity and internal consistency of the international knee documentation committee subjective knee evaluation form in children and adolescents. Am J Sports Med. 2010 Dec;38(12):2443-2447. [Pubmed]
12 Wall EJ, et al. The healing potential of stable juvenile osteochondritis dissecans knee lesions. J Bone Joint Surg Am. 2008 Dec;90(12):2655-2664. [Pubmed]
13 Peterson NS, Reeves KD. Efficacy of one day training in low-cost manual preparation of high cellular platelet rich plasma. Jnl Prolotherapy. 2014;6:922-927. [Pubmed]
14 Cahill BR, et al. The results of conservative management of juvenile osteochondritis dissecans using joint scintigraphy. A prospective study. Am J Sports Med. 1989 Sep-Oct;17(5):601-605; discussion 605-606. [Pubmed]
15 Sales de Gauzy J, et al. Natural course of osteochondritis dissecans in children. J Pediatr Orthop B. 1999 Jan;8(1):26-28. [Pubmed]
16 Hughes JA, et al. Juvenile osteochondritis dissecans: a 5-year review of the natural history using clinical and MRI evaluation. Pediatr Radiol. 2003 Jun;33(6):410-417. [Pubmed]
17 Chambers HG, et al. Diagnosis and treatment of osteochondritis dissecans. J Am Acad Orthop Surg. 2011 May;19(5):297-306. [Pubmed]
18 O’Connor MA, et al. Osteochondritis dissecans of the knee in children. A comparison of MRI and arthroscopic findings. J Bone Joint Surg Br. 2002 Mar;84(2):258-262. [Pubmed]
19 Sánchez M, et al. Plasma rich in growth factors to treat an articular cartilage avulsion: a case report. Med Sci Sports Exerc. 2003 Oct;35(10):1648-1652. [Pubmed]